NAD+, MOTS-c and Glutathione Compared
NAD+ is an oxidised dinucleotide coenzyme. MOTS-c is a 16-residue mitochondrial-derived peptide. Reduced glutathione (GSH) is a tripeptide that participates in redox chemistry. Their common appearance in metabolic research gives you a reason to compare their roles, but each material requires its own identity and measurement method. [1] [2] [3]
NAD+, MOTS-c and glutathione side by side
| Attribute | NAD+ | MOTS-c | Glutathione |
|---|---|---|---|
| Reference class | Oxidised dinucleotide coenzyme | 16-residue peptide | Redox-active tripeptide in its reduced reference form |
| Key identity distinction | Supplied form and grade | Sequence, termini and supplied form | Reduced GSH versus oxidised GSSG |
| Quantity question | Which entity the mass represents | Peptide/form basis | Form and mass basis |
| Relevant evidence question | Identity and related-species/content method | Peptide identity and content | Analyte definition and oxidation-state context |
For NAD+, establish the oxidised species and supplied form. For MOTS-c, match the sequence and terminal groups. For glutathione, distinguish GSH from glutathione disulfide (GSSG). These checks come before a comparison of catalogue amounts. [1] [2] [3]
Related pathways do not create interchangeable materials
Choose the material by the experimental question. A reaction that uses NAD+ as an electron acceptor requires a defined coenzyme. A MOTS-c experiment asks about a specified peptide and a measured cellular response. An assay of the GSH/GSSG balance must distinguish the species or state exactly what its total includes.
The guides to NAD metabolism, mitochondrial-derived peptides and glutathione redox chemistry explain the different measurements. In a methods comparison, record the analyte beside the result. “Total glutathione” and “reduced glutathione” are different entries on that worksheet.
Compare quantities only after identity is resolved
The UK catalogue lists NAD+ 1000 mg, MOTS-c 10 mg and glutathione 1500 mg. Those numbers identify nominal vial masses. To compare moles, first match the mass basis for each presentation to the molar mass of that exact chemical form.
The mass and molarity guide gives the calculation once the inputs are known. Check storage and stability evidence separately for the material, container and conditions used in your assay.
For the underlying molecular evidence, open the NAD+ profile, MOTS-c profile or glutathione profile. To verify a supplied material, request its identity, quantitative result and lot reference through batch documentation.
Follow the comparison
| Resource | Why open it |
|---|---|
| Longevity and Mitochondrial Research Compounds | Browse the ageing, mitochondrial and redox research category. |
| Compound Comparisons | Choose a comparison by its specific identity question. |
Sources and scope
- ChEBI. NAD+, CHEBI:15846. ChEBI defines the oxidised dinucleotide and its role in redox reactions.
- Lee et al. (2015). MOTS-c and metabolic homeostasis. Cell Metabolism. The original report identifies a 16-residue peptide and describes cellular and mouse experiments.
- ChEBI. Glutathione, CHEBI:16856. ChEBI identifies the reduced tripeptide and the side-chain linkage in its reference structure.